Our study suggests that manipulation of PTEN could hold promise for enhancing efficacy of differentiation therapy of acute myelogenous leukemia. At the same time, the presence of siRNA hampers accumulation of apoptotic cells during incubation. Our results show that PTEN siRNA increases HL-60 cell differentiation in the presence of DMSO and ATRA. In this study, we inhibited PTEN tumor suppressor gene expression by siRNA to investigate the effect of potentiating cell survival and inhibiting apoptosis on HL-60 cell differentiation by DMSO and ATRA. Tumor suppressor genes have a vital role in the gateway to terminal cell differentiation. A variety of chemicals stimulates differentiation of leukemic cells, such as dimethyl sulfoxide (DMSO) and all-trans retinoic acid (ATRA). Therapeutic interventions to restore terminal differentiation (''differentiation therapy'') are a very attractive way to treat cancer, especially leukemia. Thus, understanding the molecular mechanisms regulating NET formation in LPS-induced neutrophil-like cells would support the development of new therapeutic methods.Ībnormal cell differentiation, in particular suppression of terminal cell differentiation, exists in all tumors. Conclusion: Consequently, the LPS-induced infection and lethality may occur through various mechanisms. Results: Our results showed that 75% NETs was produced by HL-60 differentiated neutrophil cells exposed to 200 ng/ml LPS in 45 minues. The statistical significance of the difference between the control and treated groups was evaluated using a one-way ANOVA. Trypan blue as vital staining was used for viability. an IQTL (ImageQuant TL) converter (GE Healthcare, Sweden). Neutrophil-like cells were incubated with LPS (200 ng/ml) for 45 min, followed by incubation for 25 min with 100 ng/ml Hoechst 33342. African Journal for Physical, Health Education, Recreation and Dance.
Flow cytometry was used to evaluate CD11b in the differentiated cells, and the NBT assay was used to evaluate the functionality of the differentiated HL-60 cells. Methods: In this study, the HL60 cell line was used for culture and DMSO for induction and differentiation. This study focused on whether LPS induced NETs release in vitro in the HL60 cell line. NETs is a new strategy for pathogen response. Recently, a novel strategy called the formation neutrophil extracellular traps (NETs) was described. Background: Neutrophils are the most abundant white blood cells in humans.
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